DNA

Part:BBa_K3264011:Design

Designed by: Mia Wang   Group: iGEM19_GreatBay_SZ   (2019-10-16)


NT-BsaI-CT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 456
    Illegal BsaI.rc site found at 431


Design Notes

According this part design, it's easily to insert multiple repetition regions into NT and CT using type IIs assembly methods.


Source

de novo synthesis

References